《植物生理学报》 2013, 49(11): 1189-1196

橡胶树胶乳4个HbRab基因的克隆和表达分析

唐朝荣*, 秦云霞, 黄亚成, 方永军

中国热带农业科学院橡胶研究所, 农业部橡胶树生物学与遗传资源利用重点实验室, 海南儋州571737

通信作者：唐朝荣；E-mail: chaorongtang@126.com；Tel: 0898-23307223

摘　要：

克隆了橡胶树胶乳中表达的4个Rab基因的全长cDNA, 命名为HbRab5~HbRab8。它们编码22~24 kDa的蛋白, 均具有小G蛋白家族共有的GTP/GDP结合保守结构域, 分别属于植物Rab家族的F、D、A和B亚家族成员。组织表达分析显示, 除HbRab6外, 其他3个HbRab基因均在胶乳中表达丰度最高。在胶乳中, 伤害处理显著下调HbRab6表达而上调HbRab7表达;乙烯和水杨酸处理下调HbRab6和HbRab8的表达, 甲基茉莉酸处理上调HbRab5、HbRab7、HbRab8的表达, 细胞分裂素上调HbRab5的表达。本文结果为橡胶树胶乳再生相关Rab基因的筛选与功能阐述奠定了基础。

关键词：橡胶树; Rab; 基因克隆; 表达模式分析; 胶乳再生

收稿：2013-08-19   修定：2013-10-13

资助：国家自然科学基金(30960038)、国家“863”课题(2013AA-102605)和国家天然橡胶产业技术体系育种岗(CARS-34-GW2)。

Molecular Cloning and Expressional Analysis of Four HbRab Genes from the Latex of Hevea brasiliensis (Para Rubber Tree)

TANG Chao-Rong*, QIN Yun-Xia, HUANG Ya-Cheng, FANG Yong-Jun

Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China

Corresponding author: TANG Chao-Rong; E-mail: chaorongtang@126.com; Tel: 0898-23307223

Abstract:

In this study, the full-length cDNAs encoding four Rab small GTPases were obtained by RT-PCR from the latex of H. brasiliensis (rubber tree), and named as HbRab5–HbRab8. The four HbRab genes predicted proteins of 22–24 kDa, which had conserved GTP/GDP-binding domains typical of small GTPases. According to the phylogenetic analysis, HbRab5–HbRab8 were, respectively, clustered into the groups of F, D, A and B of the Rab family. The four HbRab genes were explored by qRT-PCR for their expressional patterns in different tissues and in the latex responding to the treatments of wounding and hormones. Except HbRab6, the other three HbRab genes showed a predominance expression in the latex. In the latex, wounding treatment markedly up-regulated the expression of HbRab7 but depressed HbRab6. The treatments of ethylene and salicylic acid decreased the expressions of HbRab6 and HbRab8. Methyl jasmonate treatment stimulated the expressions of HbRab5, HbRab7 and HbRab8, whereas the expression of HbRab5 was upregulated by cytokinin treatment. Our results are conductive to further identification and characterization of the key Rab genes involved in latex regeneration of H. brasiliensis.

Key words: Hevea brasiliensis; Rab; gene cloning; expressional analysis; latex regeneration